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Lymphoplasmacytoid lymphoma: an immunohistological study
P A Hall1, A J D'Ardenne, M A Richards
1Dept. of Histopathology, St Bartholomew's Hospital, London, U.K.
The Journal of Pathology
|November 1, 1987
Summary
This study characterized lymphoplasmacytoid lymphoma (LPL) using monoclonal antibodies, revealing B-cell marker expression and heterogeneity. Findings suggest LPL originates from non-germinal center cells and highlight CD5 expression
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Lymphoplasmacytoid lymphoma (LPL) is a distinct B-cell lymphoma.
- Immunophenotypic characterization is crucial for lymphoma diagnosis and understanding origins.
Purpose of the Study:
- To immunophenotypically characterize 18 cases of LPL using a comprehensive panel of monoclonal antibodies.
- To investigate the cellular origin and potential diagnostic markers of LPL.
Main Methods:
- Utilized a panel of 26 monoclonal antibodies for immunophenotypic analysis.
- Assessed expression of B-cell markers (CD19, CD20, CD22), MHC class II, cytoplasmic and surface immunoglobulins, and proliferation markers (Ki67).
- Analyzed the association of CD5 expression with clinical features like peripheral blood lymphocytosis.
Main Results:
- All LPL cases expressed leukocyte common antigen, MHC class II, and B-cell markers, with variable loss of some markers.
- Phenotypic heterogeneity was observed, including variable expression of KB61, CD21, and CD5.
- Cytoplasmic immunoglobulin was present in all cases; surface immunoglobulin light chain restriction and heavy chain predominance were detected in most.
- A significant association was found between CD5 expression and peripheral blood lymphocytosis (p < 0.003).
Conclusions:
- Data supports a non-germinal center cell origin for LPL.
- CD5 expression on B cells in LPL may be linked to lymphocyte migration.
- LPL exhibits immunological heterogeneity, posing diagnostic challenges but requiring recognition due to its poor prognosis.