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Updated: Jan 23, 2026

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
Published on: April 9, 2017
Super-resolution Microscopy of Vaccinia Virus Particles
1MRC Laboratory for Molecular Cell Biology, University College London, London, UK. robert.gray.14@ucl.ac.uk.
Abstract:
Super-resolution microscopy enables the study of vaccinia architecture at subviral resolution with molecular specificity. Here, we outline how to use structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) to detect fluorescently tagged or immunolabeled viral proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle averaging or quantitative analysis of viral protein spatial organization.
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