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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Published on: April 14, 2010
Protein inhibitor of activated STAT genes are differentially expressed in breast tumor tissues
Mohammad Taheri1,2, Vahid K Oskooei3, Soudeh Ghafouri-Fard3
1Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:
Aim: Protein inhibitor of activated STAT (PIAS) family includes transcriptional regulator proteins with SUMO E3 ligase activity. They regulate expression of several genes involved in cell proliferation, differentiation and survival. Method: We evaluated expression of PIAS1-4 genes in 54 breast cancer tissues and their paired adjacent noncancerous tissues. Results:PIAS2 and PIAS3 genes were significantly downregulated in tumoral tissues compared with adjacent noncancerous tissues. PIAS1-3 expressions were significantly lower in estrogen receptor (ER+) samples compared with ER- samples while PIAS4 had the opposite trend. PIAS3 expression was significantly higher in grade 1 samples compared with grade 2 samples. Conclusion: These findings highlight the role of PIAS genes in the pathogenesis of breast cancer and their association with determinants of response to antihormone therapies.
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