Related Experiment Video
Updated: Jan 22, 2026

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
Published on: September 6, 2018
Generating tissue-engineered intestinal epithelium from cultured Lgr5 stem cells in vivo
Zhassulan Baimakhanov1, Yasuhiro Torashima1, Akihiko Soyama1
1Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
Introduction:
Generating tissue-engineered small intestine (TESI) from mature intestinal cells has been established in a mouse model. The purpose of this study was to generate TESI from Lgr5 stem cells in vivo.
Methods:
We used Lgr5-EGFP mice for intestinal crypt isolation. After seven days, cultured crypts with Lgr5 stem cells were seeded onto a biodegradable polymer and implanted into omentum of NOD/SCID mice.
Results:
Engineered intestinal epithelium was generated from Lgr5 stem cells after four weeks of in vivo implantation. Intestinal epithelium was immunohistochemically positive for Paneth cells, enteroendocrine cells, goblet cells, microvilli of the absorptive enterocytes and Ki67.
Conclusion:
Our observations suggest that transplanted Lgr5 stem cells can differentiate into the intestinal epithelium in vivo with further proliferative activity.
Related Concept Videos
Adult Stem Cells
Renewal of Intestinal Stem Cells
Stem Cell Culture
Classification of Epithelial Tissues: Simple Epithelium
Because of the thinness of the cells, simple squamous epithelium is present where the rapid passage of chemical compounds is observed. For example, the endothelium that lines the capillaries and vessels...
Tissue Renewal without Stem Cells
However, failure of such a system...
Plant Tissue Culture

