Related Experiment Video
Updated: Jan 22, 2026

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice
Published on: August 30, 2017
[Effects of H102 on the memory recognition ability and AMPK-mTOR autophagy-related pathway in AD mice]
Shang Ran Shan1, Fang Jiang1, Shu Mei Xu1
1Department of Physiology, Tianjin Medical University, Tianjin 300070, China.
Objective:
To study the effect of β-sheet blocking peptide H102 on the expression of AMPK-mTOR autophagy pathway-related protein in APP/PS1 double transgenic AD mice.
Methods:
Thirty male APP/PS1 transgenic male AD mice of 6 months old were randomly divided into AD group and H102 intervention group, and C57BL/6J male mice of the same age were used as control group (n=15). The mice in the HF group were administered with 5 μl (5.8 mg/kg) of H102 polypeptide solution through the nasal cavity at the same time period, and the mice in the control group and the AD group were given the same amount of blank adjuvant solution daily. The memory recognition ability was tested by a new object recognition experiment 30 days after continuous administration. Immunohistochemistry and Western blot were used to detect the expressions of phosphorylated AMP-activated protein kinase(P-AMPK),phosphorylated mammalian target of rapamycin (P-mTOR) and ratio of LC32to LC31(LC3II/I )in brain tissue.
Results:
Compared with the control group, the new object recognition index (RI) of the AD group was significantly lower (P<0.05), and the P-AMPK and LC3II/I ratios in the brain of the mice were significantly lower (P<0.05). The expression of P-mTOR protein was increased significantly (P<0.05). Compared with the AD group, the RI of the H102 intervention group was increased significantly (P<0.05), and the P-AMPK and LC3II/I ratios in the brain tissue of the mice were increased significantly (P<0.05). The expression of P-mTOR protein was decreased significantly (P<0.05).
Conclusion:
H102 can improve the recognition and memory ability of AD mice by activating the AMPK-mTOR autophagy-related pathway.
Related Concept Videos
PI3K/mTOR/AKT Signaling Pathway
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Autophagy
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
System of Memory
Working Memory

