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Updated: Jan 22, 2026

Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts
Published on: May 14, 2021
Immune function of an angiotensin-converting enzyme against Rice stripe virus infection in a vector insect
Xue Wang1, Wei Wang2, Wenzhong Zhang3
1College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
Abstract:
Angiotensin-converting enzyme (ACE) plays diverse roles in the animal kingdom. However, whether ACE plays an immune function against viral infection in vector insects is unclear. In this study, an ACE gene (LsACE) from the small brown planthopper was found to respond to Rice stripe virus (RSV) infection. The enzymatic activities of LsACE were characterized at different pH and temperature. Twenty planthopper proteins were found to interact with LsACE. RSV infection significantly upregulated LsACE expression in the testicle and fat body. When the expression of LsACE in viruliferous planthoppers was inhibited, the RNA level of the RSV SP gene was upregulated 2-fold in planthoppers, and all RSV genes showed higher RNA levels in the rice plants consumed by these planthoppers, leading to a higher viral infection rate and disease rating index. These results indicate that LsACE plays a role in the immune response against RSV transmission by planthoppers.
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