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Rat kidney mitochondrial carbonic anhydrase.

S J Dodgson1, L C Contino

  • 1Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104-6085.

Archives of Biochemistry and Biophysics
|January 1, 1988
PubMed
Summary

Mitochondrial carbonic anhydrase was detected in rat kidney mitochondria, unlike in guinea pigs. This enzyme

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Enzymology

Background:

  • Mitochondrial carbonic anhydrase (CA) presence and function are not fully understood.
  • Previous studies detected CA in liver mitochondria but not guinea pig kidney mitochondria.

Purpose of the Study:

  • To investigate the presence and activity of carbonic anhydrase in rat kidney mitochondria.
  • To compare mitochondrial CA activity across different physiological conditions and species.

Main Methods:

  • Enzyme activity assays using the 18O-labeling technique to measure CO2 hydration rates.
  • Electron microscopy to confirm mitochondrial integrity.
  • pH and bicarbonate concentration dependency studies.

Main Results:

  • Carbonic anhydrase activity was confirmed within the inner mitochondrial membrane of rat kidney mitochondria.
  • Mitochondrial CA activity varied with diet (acidic diet increased activity) and species (present in rats, absent in guinea pigs).
  • Rat liver and kidney mitochondrial CA showed similar kinetic properties and pH dependency.

Conclusions:

  • Rat kidney mitochondria possess active carbonic anhydrase, likely located within the inner mitochondrial membrane.
  • The presence of mitochondrial CA in rats, but not guinea pigs, may explain differences in kidney gluconeogenesis.
  • Rat liver and kidney mitochondrial CA are likely the same isozyme.

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