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Updated: Jan 22, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes
Published on: May 4, 2020
Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS)
Glenn R Masson1, John E Burke2, Natalie G Ahn3
1MRC Laboratory of Molecular Biology, Cambridge, UK. gmasson@mrc-lmb.cam.ac.uk.
None:
Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.
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