Related Experiment Video
Updated: Jan 22, 2026

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
Published on: March 9, 2017
Mammalian synthetic biology by CRISPRs engineering and applications
Kenta Katayama1, Hitoshi Mitsunobu1, Keiji Nishida1
1Engineering Biology Research Center, Kobe University, 7-1-49, Minatojima Minami Machi, Chuo-ku, Kobe 650-0047, Japan.
Abstract:
Technologies harnessing CRISPR systems have been rapidly evolving and expanding the capacity of researchers for understanding of mammalian cell behavior and its underlying mechanisms through genome and epigenome manipulations. In this review, we summarized the recent developments of CRISPR-based technologies for genetic and epigenetic modifications that include engineering of Cas9 for PAM simplification, non-cleaving base editing tools and alteration of gene expression. Applications such as genome-wide screening methods or CRISPR-based DNA barcoding for cellular lineage tracking are highlighted. Anticipated and upcoming development for mammalian synthetic biology that includes organelle engineering is also discussed.
Related Concept Videos
Synthetic Biology
Golden rice
Golden rice is a genetically modified...
Applications Of NMR In Biology
CRISPR
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
What is Genetic Engineering?
What is Conservation Biology?

