Optimization of nitrogen removal performance in a single-stage SBR based on partial nitritation and ANAMMOX

A partial nitritation (PN)/anaerobic ammonium oxidation (ANAMMOX) process in sequencing batch reactor (SBR) was successfully developed to treat high-strength ammonium wastewater. The feed distribution in the SBR cycle and sub-cycles was considered as the main operating strategy, and was optimized using a response surface methodology (RSM)-based optimization technique. In the SBR cycle, the maximum nitrogen removal rate (NRR) of 0.79 ± 0.01 kg m^-3 d^-1 was achieved by applying a feed distribution strategy that considered the kinetic characteristics of ANAMMOX and ammonia oxidizing bacteria (AOB). However, this strategy negatively affected the nitrogen removal efficiency (NRE) due to alkalinity loss. Therefore, the feed distribution in the SBR sub-cycles with respect to the NRE and the NRR was further studied. The nitrogen removal performance was optimized in the optimum region and an NRE of 88% and an NRR of 0.84 kg m^-3 d^-1 were achieved. The optimized model was verified in confirmation test. The RSM-based optimization results provide insights into the feed distribution strategy for achieving single-stage PN/ANAMMOX SBR operation.