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Updated: Jan 22, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
07:16

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

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[The effects of tectochrysin on prostate cancer cells apoptosis and its mechanism]

Yu Wang1, Rui-Jun Ke1, Pan-Ruo Jiang

  • 1Shaoxing University School of Medicine, Shaoxing 312000, China.

Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology
|July 2, 2019
PubMed
Abstract

Insights

Tectochrysin inhibits prostate cancer cell proliferation and induces apoptosis by modulating TRAIL and PI3K/AKT pathways. This natural compound demonstrates potential as an anti-prostate cancer agent.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Prostate cancer remains a significant health concern globally.
  • Identifying novel therapeutic agents is crucial for effective treatment.
  • Tectochrysin, a natural flavonoid, has shown potential anti-cancer properties.

Purpose of the Study:

  • To investigate the anti-cancer effects of tectochrysin on the 22Rv.1 prostate cancer cell line.
  • To elucidate the molecular mechanisms underlying tectochrysin's action.
  • To evaluate tectochrysin's efficacy in an in vivo model.

Main Methods:

  • Cell proliferation was assessed using MTS assay.
  • Apoptosis, cell cycle, and nuclear changes were analyzed via flow cytometry and Hoechst staining.
  • Cytotoxicity was determined by LDH release assay.
  • Gene expression changes were analyzed using qPCR and Western blot.
  • In vivo anti-tumor effects were evaluated in BALB/c mice.

Main Results:

  • Tectochrysin significantly inhibited 22Rv.1 cell proliferation and induced apoptosis.
  • Upregulation of pro-apoptotic genes (DR4, DR5, TRAIL, p53, caspases, BAX, FOXO3) and downregulation of anti-apoptotic genes (AKT, PI3K, BCL-2) were observed.
  • Tectochrysin demonstrated anti-tumor effects in vivo.

Conclusions:

  • Tectochrysin induces apoptosis in prostate cancer cells.
  • The mechanism involves modulation of the TRAIL and PI3K/AKT signaling pathways.
  • Tectochrysin exhibits anti-prostate cancer activity.

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