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Related Experiment Videos

Isolation of type 5 capsular polysaccharide from Staphylococcus aureus.

J M Fournier1, K Hannon, M Moreau

  • 1Unité du Choléra et des Vibrions, Institut Pasteur, Paris.

Annales De L'Institut Pasteur. Microbiology
|September 1, 1987
PubMed
Summary

Staphylococcus aureus clinical isolates commonly feature type 5 and type 8 capsular polysaccharides. This study isolated and characterized type 5 capsular polysaccharide, confirming its distinct chemical and immunological properties.

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Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • Clinical surveys reveal Staphylococcus aureus frequently exhibits type 5 and type 8 capsular polysaccharides.
  • Understanding the distinct properties of these polysaccharides is crucial for developing targeted therapies.

Purpose of the Study:

  • To isolate and chemically characterize the type 5 capsular polysaccharide from clinical Staphylococcus aureus isolates.
  • To determine the immunological and chemical distinctness of type 5 capsular polysaccharide compared to type 8 and teichoic acid.

Main Methods:

  • Extraction of type 5 capsular polysaccharide from S. aureus grown on solid media.
  • Purification using ion exchange chromatography and gel filtration.
  • Chemical analysis (composition) and immunological assays (immunodiffusion, 13C NMR).

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Main Results:

  • Type 5 capsular polysaccharide comprises N-acetylfucosamine and N-acetylhexosaminuronic acid.
  • Demonstrated immunological and chemical differences between type 5 and type 8 capsular polysaccharides.
  • Confirmed distinctness from Staphylococcus aureus teichoic acid.

Conclusions:

  • The type 5 capsular polysaccharide of Staphylococcus aureus has a unique chemical structure.
  • Type 5 capsular polysaccharide is immunologically and chemically distinct from type 8 capsular polysaccharide and teichoic acid.
  • These findings contribute to the understanding of Staphylococcus aureus virulence factors.