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Updated: Aug 4, 2026
Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
Published on: October 9, 2013
Supervised learning to quantify amyloidosis in whole brains of an Alzheimer's disease mouse model acquired with
David Nguyen1,2,3, Virginie Uhlmann4,5, Arielle L Planchette1,3
1Laboratory of Nanoscale Biology, École Polytechnique Fédérale de Lausanne, Lausanne, Vaud, Switzerland.
Abstract:
Alzheimer's disease (AD) is characterized by amyloidosis of brain tissues. This phenomenon is studied with genetically-modified mouse models. We propose a method to quantify amyloidosis in whole 5xFAD mouse brains, a model of AD. We use optical projection tomography (OPT) and a random forest voxel classifier to segment and measure amyloid plaques. We validate our method in a preliminary cross-sectional study, where we measure 6136 ± 1637, 8477 ± 3438, and 17267 ± 4241 plaques (AVG ± SD) at 11, 17, and 31 weeks. Overall, this method can be used in the evaluation of new treatments against AD.
