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Published on: October 13, 2016
INHALATORY ISOFLURANE ANESTHESIA IN MOUNTAIN CHICKEN FROGS (LEPTODACTYLUS FALLAX)
Alberto Rodriguez Barbon1, Andrew Routh2, Javier Lopez3
1Durrell Wildlife Conservation Trust, Les Augrès Manor, La Profonde Rue, Trinity, Jersey, JE3 5BP, Channel Islands, alberto.barbon@durrell.org.
Abstract:
One hundred and fourteen mountain chicken frogs were anesthetized, to place intracoelomic radiotracers. The animals were placed in a clear plastic bag that was filled with isoflurane 5% and oxygen. Loss of righting reflex occurred at 3.4 ± 2.3 min; loss of gular movements was observed at 7.6 ± 2.7 min. Intubation was carried out using a modified cuffed tube between 2.5 and 3.5 mm, at 7.6 ± 2.2 min from the beginning of the anesthesia. Manual intermittent positive pressure ventilation every 5 to 10 sec was initiated and maintained through the anesthesia. Isoflurane concentration was maintained at 2%. Loss of withdrawal reflex occurred at 10.6 ± 4.8 min, while loss of response to painful stimuli was noted at 11.1 ± 2.9 min. Surgery started at 16.9 ± 9.9 min; the procedure from incision to last suture took 8.2 ± 2.3 min. Total anesthesia time was 21 ± 6.4 min. Intermittent positive pressure ventilation was continued with room air until the animals recovered the righting reflex, which occurred at 40.4 ± 10.1 min.
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