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Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions
Published on: November 6, 2016
Isolation, qualitative and quantitative evaluation of galactomannan during germination of Trigonella persica
Ehsan Bakhshy1, Fatemeh Zarinkamar1, Mehrdad Nazari1
1Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract:
Galactomannan extracted from Trigonella persica endosperm like to other galactomannans is useful in the medicine and pharmaceutical industry such as drug delivery due to its unique physicochemical properties. As soon as germination takes place, the hydrolysis of galactomannan begins and its structure changes. Evaluation of galactomannan content extracted from the germinating seeds (GEGS) and the non-germinated seeds (GENGS) showed that the amount of galactomannan was highest at 24 h after imbibition and was completely consumed approximately 48 h after imbibition. It seems that galactomannan is used for storage reserve synthesis in the cotyledon during germination. Based on the quantitative assessment, FT-IR, macroscopic and microscopic observations, galactomannan of T. persica seeds have high an M/G ratio (approximately 5:1) and this ratio does not alter during germination. Therefore, changes in galactomannan structure (functional group and band) caused that GEGS have different physicochemical properties than GENGS. Based on the results, GENGS with a high M/G ratio may be suitable for drug delivery system due to some properties such as high viscosity and gelling.
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