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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Chromatin Packaging02:21

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Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
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Chromatin Packaging01:32

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Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
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The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
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Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
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Related Experiment Video

Updated: Jan 22, 2026

Test Samples for Optimizing STORM Super-Resolution Microscopy
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Super-Resolution Microscopy of Chromatin.

Udo J Birk1,2

  • 1University of Applied Sciences HTW Chur, Pulvermühlestrasse 57, 7004 Chur, Switzerland. udo.birk@htwchur.ch.

Genes
|July 3, 2019
PubMed
Summary

Super-resolution microscopy and chromatin labeling reveal gene regulation insights. These techniques allow direct assessment of chromatin interactions and conformational states for better cellular understanding.

Keywords:
DNADNA labelingchromatinfluorescencenuclear architecturesingle molecule localization microscopysuper-resolution microscopy

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Area of Science:

  • Cell Biology
  • Genetics
  • Microscopy

Background:

  • Super-resolution microscopy has advanced cellular process understanding.
  • Chromatin-specific fluorescence labeling aids gene regulation and organization studies.

Purpose of the Study:

  • To explore how super-resolution imaging combined with chromatin labeling enhances insights into gene regulation and chromatin organization.
  • To enable direct assessment of chromatin interactions and conformational states.

Main Methods:

  • Utilizing super-resolution microscopy techniques.
  • Employing chromatin-specific fluorescence labeling.
  • Applying advanced data analysis.

Main Results:

  • Gained deeper insights into gene regulation mechanisms.
  • Achieved better understanding of chromatin organization.
  • Enabled direct assessment of chromatin interactions.
  • Evaluated the function of specific chromatin conformational states.

Conclusions:

  • Super-resolution microscopy coupled with specific labeling techniques provides powerful tools for studying chromatin dynamics.
  • These integrated approaches significantly advance our comprehension of cellular processes at a high resolution.