Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lamin B1 safeguards the B cell genome and shapes lymphoma outcome.

HemaSphere·2026
Same author

Single-molecule localization microscopy imaging of extracellular vesicle DNA in recipient cells.

Journal of translational medicine·2026
Same author

A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context.

bioRxiv : the preprint server for biology·2025
Same author

Cell aging - a relevant factor in live cell microscopy (mini-review).

Progress in biophysics and molecular biology·2025
Same author

Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.

Journal of cell science·2025
Same author

Perspective Article: Space-time dynamics of genome replication studied with super-resolved microscopy.

Postepy biochemii·2024

Related Experiment Video

Updated: Jul 10, 2026

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Nanostructure analysis using spatially modulated illumination microscopy.

David Baddeley1, Claudia Batram, Yanina Weiland

  • 1Kirchhoff Institut für Physik, Universität Heidelberg, INF 227, D-69120 Heidelberg, Germany. baddeley@kip.uni-heidelberg.de

Nature Protocols
|October 20, 2007
PubMed
Summary

Spatially modulated illumination microscopy visualizes cellular nanostructures. This protocol details sample preparation, data acquisition, and analysis for accurate size and position estimation.

More Related Videos

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Related Experiment Videos

Last Updated: Jul 10, 2026

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Area of Science:

  • Microscopy and Imaging
  • Cell Biology
  • Nanotechnology

Background:

  • Accurate characterization of cellular nanostructures is crucial for understanding biological processes.
  • Traditional microscopy techniques may lack the resolution required for nanoscale analysis.
  • Spatially Modulated Illumination (SMI) microscopy offers enhanced resolution for nanoscale imaging.

Purpose of the Study:

  • To provide a comprehensive protocol for using Spatially Modulated Illumination (SMI) microscopy.
  • To enable accurate estimation of sizes and positions of fluorescently labeled cellular nanostructures.
  • To guide researchers in sample preparation, data acquisition, and evaluation using SMI microscopy.

Main Methods:

  • Detailed description of the Spatially Modulated Illumination (SMI) microscope setup and handling.
  • Step-by-step protocol for sample preparation, focusing on cells attached to cover glass.
  • Guidelines for data acquisition and subsequent analysis of nanostructure properties.

Main Results:

  • Demonstration of SMI microscopy's capability for precise nanostructure size and position determination.
  • The protocol is optimized for fixed specimens but adaptable for faster in vivo measurements.
  • Successful application of SMI for analyzing fluorescently labeled cellular components.

Conclusions:

  • Spatially Modulated Illumination (SMI) microscopy is a valuable tool for nanoscale cellular analysis.
  • The presented protocol facilitates reliable characterization of cellular nanostructures.
  • SMI microscopy enables both fixed and in vivo studies of nanostructures.