Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lamin B1 safeguards the B cell genome and shapes lymphoma outcome.

HemaSphere·2026
Same author

MeCP2 NID interaction with RNA: implications for Rett syndrome-relevant protein regulation.

Human molecular genetics·2026
Same author

TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory network.

Nature communications·2026
Same author

Single-molecule localization microscopy imaging of extracellular vesicle DNA in recipient cells.

Journal of translational medicine·2026
Same author

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy.

Journal of visualized experiments : JoVE·2025
Same author

Benchmarking porcine pancreatic ductal organoids for drug screening applications.

EMBO molecular medicine·2025
Same journal

Fungal secondary metabolites as a source of bioactive compounds: a modern approach to their acquisition and analysis

Postepy biochemii·2026
Same journal

140 years of the model organism Escherichia coli as the "workhorse of molecular biology and biomedicine"

Postepy biochemii·2026
Same journal

The Year 2026 in Biology: A Jubilee Year

Postepy biochemii·2026
Same journal

New possibilities and perspectives for the use of fly maggots in clinical practice

Postepy biochemii·2026
Same journal

Laccase as a useful biotechnological tool in the synthesis of biologically active compounds

Postepy biochemii·2026
Same journal

Methodological aspects of determining flavonoids in food products with cardioprotective potential – a review of HPLC conditions

Postepy biochemii·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

22.5K

Perspective Article: Space-time dynamics of genome replication studied with super-resolved microscopy.

Marton Gelleri1, Michael Sterr2, Hilmar Strickfaden3

  • 1Institute of Molecular Biology (IMB), 55128 Mainz, Germany.

Postepy Biochemii
|July 17, 2024
PubMed
Summary
This summary is machine-generated.

Genome replication shows a distinct spatial pattern, with newly synthesized DNA concentrating in the inactive nuclear compartment. This challenges current models of nuclear organization and replication dynamics.

More Related Videos

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

18.7K
Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.5K

Related Experiment Videos

Last Updated: May 10, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

22.5K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

18.7K
Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.5K

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Genome replication necessitates duplicating DNA, nucleosomes, and epigenetic marks.
  • Despite advances, key challenges in genome replication persist, including accessibility and maintaining chromatin structure.

Purpose of the Study:

  • To investigate the spatial distribution of newly replicated DNA within the nucleus.
  • To explore the relationship between DNA replication and nuclear architecture.

Main Methods:

  • EdU pulse-labeling and chase experiments in mouse myeloblast cells.
  • 3D structured illumination microscopy (SIM) for nuclear imaging.
  • Analysis of DNA intensity classes as proxies for chromatin compaction.

Main Results:

  • Newly replicated DNA was under-represented in the active nuclear compartment (low DNA density) and over-represented in the inactive nuclear compartment (high DNA density) immediately after labeling.
  • This shift towards higher DNA density classes became more pronounced after a chase period.
  • Findings contrast with established transcriptional topography, which shows active marks in low-density regions.

Conclusions:

  • Replication dynamics exhibit a distinct spatial segregation within the nucleus, favoring condensed regions.
  • Current models of chromatin domains (CDs) as frameworks or phase-separated droplets may not fully explain these observations.
  • Methodological limitations hinder the integration of replication and transcription spatial data into a unified model of nuclear architecture.