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Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

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Related Experiment Video

Updated: Jan 12, 2026

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

Published on: March 30, 2017

Cell aging - a relevant factor in live cell microscopy (mini-review).

Tina Karimian¹, Christoph Cremer², Julian Weghuber³

¹University of Applied Sciences Upper Austria, 4600, Wels, Austria.

Progress in Biophysics and Molecular Biology

|November 1, 2025

Summary

This summary is machine-generated.

Cell aging alters cellular structures and functions, impacting live-cell microscopy results. Researchers must consider cell passage number and senescence markers for accurate experimental design and data interpretation.

Keywords:

Fluorescence microscopy Light microscopy Living cells Senescence cell passage number

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Last Updated: Jan 12, 2026

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Area of Science:

Cell Biology
Microscopy Techniques

Background:

Live-cell microscopy is crucial for studying cellular responses to environmental stimuli.
Cell aging induces significant changes in cellular structures and functions, potentially distorting microscopy data.

Purpose of the Study:

To review how cell aging affects subcellular compartments and live-cell imaging outcomes.
To focus on the impact of cell aging on advanced microscopy and micromanipulation techniques.

Main Methods:

Literature review focusing on cell aging's effects on microscopy.
Analysis of changes in gene expression, nuclear architecture, metabolism, and mechanical properties.
Examination of advanced techniques: super-resolution, FLIM, VA-TIRFM, and laser-assisted optoporation.

Main Results:

Cell aging modifies critical subcellular compartments, influencing live-cell imaging.
Aging impacts the performance and outcomes of advanced microscopy and micromanipulation.
Specific changes affect nuclear architecture, energy metabolism, and cell membrane mechanics.

Conclusions:

Cellular aging significantly affects live-cell microscopy readouts.
Experimental design and data interpretation must account for cell passage number and senescence.
Understanding aging effects is vital for accurate live-cell imaging studies.