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Monitoring Nanoparticle Interaction with Murine Breast Cancer Cells Using Multimodal Fluorescence Lifetime

Steven Eckstein1, Louisa Herbsleb1, Henriette Gröger2

  • 1Center for Optical Technologies, Aalen University, Beethovenstr. 1, 73430 Aalen, Germany.

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|February 13, 2026
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Summary
This summary is machine-generated.

This study shows how fluorescence lifetime measurements can track inorganic-organic hybrid nanoparticles (IOH-NPs) in breast cancer cells. This technique reveals nanoparticle location and aids cancer therapy development.

Keywords:
FRETcellular locationfluorescence lifetimesinorganic–organic hybrid nanoparticles (IOH-NPs)light microscopy

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Therapy

Background:

  • Understanding nanoparticle uptake is crucial for effective cancer drug delivery.
  • Current microscopy techniques have limitations in resolving nanoscale cellular localization.

Purpose of the Study:

  • To investigate the cellular uptake and localization of inorganic-organic hybrid nanoparticles (IOH-NPs) in breast cancer cells.
  • To explore the utility of fluorescence lifetime measurements as a novel tool for studying nanoparticle-cell interactions.

Main Methods:

  • Integration of fluorescence lifetime measurements, microspectrometry, and confocal laser scanning microscopy.
  • Tracking IOH-NPs in breast cancer cells over 2-24 hour incubation periods.
  • Utilizing Förster Resonance Energy Transfer (FRET) between LysoTracker Green and IOH-NPs.

Main Results:

  • Confirmed lysosomal localization of IOH-NPs through FRET.
  • Demonstrated that fluorescence lifetime kinetics can reveal nanoscale cellular localization beyond traditional microscopy limits.
  • Showcased picosecond fluorescence decay times as a fifth dimension complementing spatial and spectral data.

Conclusions:

  • Fluorescence lifetime measurements offer a versatile approach to study nanoparticle uptake in cancer therapy.
  • This technique can guide the optimization of nanoparticle design and fluorescence excitation for improved cancer treatment.
  • Extended optical microscopy capabilities for nanoscale cellular analysis.