Related Experiment Video
Updated: Jan 22, 2026
08:15
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
Published on: October 8, 2016
7.8K
Comparison of Human Tissue Microarray to Human Pericyte Transcriptome Yields Novel Perivascular Cell Markers
Ching Yun Hsu1, Mario Gomez Salazar2,3, Sarah Miller1
1Department of Pathology, Johns Hopkins University, Baltimore, Maryland.
Stem Cells and Development
|July 3, 2019
Summary
Researchers identified novel protein markers for human pericytes, crucial multipotent cells. These new pericyte antigens, some maintained in culture, aid in cell identification and characterization for future research.
Area of Science:
- Cell Biology
- Tissue Engineering
- Immunology
Background:
- Human perivascular progenitor cells, including pericytes, are multipotent mesenchymal cells.
- Specific antigens to distinguish pericytes from other cell types are limited.
- Pericytes reside in unique perivascular niches within various tissues.
Purpose of the Study:
- To identify novel protein markers specific to human pericytes in adipose tissue.
- To validate these markers against pericyte transcriptomic data.
- To assess the stability of marker expression in cultured pericytes.
Main Methods:
- Utilized a human tissue microarray (Human Protein Atlas) for protein expression analysis.
- Cross-referenced protein data with RNA sequencing of fluorescence-activated cell sorting (FACS) purified CD146+CD34-CD31-CD45- pericytes.
- Analyzed gene and protein expression in cultured pericytes.
Main Results:
- Identified 229 proteins with strong, specific perivascular immunoreactivity in soft tissues.
- Discovered 18 novel antigens and 7 previously known antigens consistently expressed in pericytes.
- Found that 56% of identified transcripts maintained expression after culture propagation.
- Noted that many novel antigens are regulators of osteogenic differentiation.
Conclusions:
- This study successfully identified novel pericyte-specific antigens.
- Some identified markers are conserved in cultured pericytes, useful for isolation and characterization.
- The novel antigens, particularly those involved in osteogenic differentiation, offer new avenues for pericyte research.
Related Concept Videos
01:31ATP Yield
78.4K
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle).
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
78.4K
01:24Biodiversity and Human Values
16.4K
Human civilization relies on biodiversity in many ways. Sudden changes in species biodiversity result in environmental changes that can modify weather patterns and therefore human civilizations.
16.4K
02:22Reaction Yield
59.3K
The theoretical yield of a reaction is the amount of product estimated to form based on the stoichiometry of the balanced chemical equation. The theoretical yield assumes the complete conversion of the limiting reactant into the desired product. The amount of product that is obtained by performing the reaction is called the actual yield, and it may be less than or (very rarely) equal to the theoretical yield.
59.3K
02:57The Sense of Self: Reflected Self-Appraisal and Social Comparison
55.5K
According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
55.5K
02:34DNA Microarrays
20.8K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.8K
01:28Human Genetics
1.5K
Human genetics provides a profound framework for understanding the interplay between genetic predispositions and human psychology. At the heart of this discipline lies the study of how genes influence physical traits, behaviors, and susceptibility to diseases. Each person carries a unique genetic code that subtly or significantly shapes their psychological and behavioral landscape.
The complex relationship between genetics and psychology is observable through common biological components such...
The complex relationship between genetics and psychology is observable through common biological components such...
1.5K