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Updated: Jan 22, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Updates on the Current Technologies for microRNA Profiling
Rebecca Mathew1, Valentina Mattei1, Muna Al Hashmi1
1Omics Core and Biorepository, Sidra Medicine, Out Patient Clinic, Doha, Qatar.
Abstract:
MicroRNAs are RNA molecules of ~22 nt length that regulate gene expression posttranscriptionally. The role of miRNAs has been reported in many cellular processes including apoptosis, cell differentiation, development and proliferation. The dysregulated expression of miRNAs has been proposed as a biomarker for the diagnosis, onset and prognosis of human diseases. The utility of miRNA profiles to identify and discriminate patients from healthy individuals is highly dependent on the sensitivity and specificity of the technologies used for their detection and the quantity and quality of starting material. In this review, we present an update of the current technologies for the extraction, QC assessment and detection of miRNAs with special focus to the most recent methods, discussing their advantages as well as their shortcomings.
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