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Purification of ribonuclease T1
C N Pace1, G R Grimsley, B J Barnett
1Department of Biochemistry, Texas A&M University, College Station 77843.
Analytical Biochemistry
|December 1, 1987
Abstract:
An improved method for purifying ribonuclease T1 from Aspergillus oryzae is described. The method uses gradient elution from DEAE-cellulose and sulfopropyl-Sephadex columns followed by gel filtration on Sephadex G-50 to give almost 100 mg (50% yield) of ribonuclease T1 from 100 g of starting material in less than 5 days.