Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Complex Ions03:45

Formation of Complex Ions

25.8K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
25.8K
Crossover Experiments01:16

Crossover Experiments

4.5K
Crossover experiments, also called the repeated-measurements design, is a study design in which all experimental units are exposed to all treatments in different periods. Crossover experiments are generally used in psychology, the pharmaceutical industry, agriculture, and medicine.
Crossover designs are performed even with smaller sample sizes since the samples can act as their controls. These are better than simple randomized trials since patients are exposed to all the treatments.
4.5K
RNA Interference01:23

RNA Interference

27.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.9K
Interference and Diffraction02:18

Interference and Diffraction

51.9K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
51.9K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.8K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.8K
Positive Regulator Molecules01:45

Positive Regulator Molecules

134.7K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
134.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Assessment of Sample Preparation Workflows and Mass Spectrometry Acquisition Strategies for Bottom-Up Proteomics in Rice Tissues.

Journal of agricultural and food chemistry·2026
Same author

Supercritical CO<sub>2</sub>-Decellularized amniotic ECM hydrogel promotes immunomodulatory skin regeneration.

Materials today. Bio·2026
Same author

IL-27-mediated hematopoietic dysregulation exacerbates disease severity in severe fever with thrombocytopenia syndrome virus infection.

Science translational medicine·2026
Same author

Impaired Neuregulin 1 Processing is Associated with Synaptic and Behavioral Abnormalities in a Prenatal Valproic Acid Model of Autism.

International journal of biological sciences·2026
Same author

Modulation of Host Innate Immune Response by Highly Pathogenic Human Coronaviruses during Viral Infection.

Journal of microbiology and biotechnology·2026
Same author

The insulin receptor inhibitor BMS-754807 alleviates neuroinflammation and Alzheimer's disease pathologies across human cellular and mouse models.

Journal of neuroinflammation·2026
Same journal

CYSTEINE-RICH RLK2 regulates development via callose synthase-dependent symplastic transport in Arabidopsis.

Plant physiology·2026
Same journal

H2O2 oxidation of VvMYB APL reduces VvHSP20-43 expression and promotes grape ripening.

Plant physiology·2026
Same journal

Mitigating Constraints in Harvest Index and Yield of Densified Populations via Sink Modulation of Narrowing Pollination Time Gaps within Maize Ear.

Plant physiology·2026
Same journal

The MrHY5-mru-miR396-MrGRF4 module regulates UV-B-induced quercetin biosynthesis in Chinese bayberry (Morella rubra cv. Biqi).

Plant physiology·2026
Same journal

The transcription factor StC3H14 enhances cold tolerance through the CBF-dependent pathway in potato.

Plant physiology·2026
Same journal

Jasmonic acid and PpeMYC2 regulate peach fruit ripening by controlling polyamine levels and anthocyanin biosynthesis.

Plant physiology·2026
See all related articles

Related Experiment Video

Updated: Jan 22, 2026

Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates
12:03

Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates

Published on: February 24, 2011

133.4K

A Multiprotein Complex Regulates Interference-Sensitive Crossover Formation in Rice.

Jie Zhang1, Chong Wang1, James D Higgins2

  • 1Joint International Research Laboratory of Metabolic and Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 20040, China.

Plant Physiology
|July 4, 2019
PubMed
Summary
This summary is machine-generated.

In rice, the SHORTAGE OF CHIASMATA 1 (OsSHOC1) and PARTING DANCERS (OsPTD) proteins are crucial for meiotic crossover (CO) formation. These proteins interact with other ZMM proteins, forming complexes essential for CO development.

More Related Videos

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

841
A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
08:04

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

12.6K

Related Experiment Videos

Last Updated: Jan 22, 2026

Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates
12:03

Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates

Published on: February 24, 2011

133.4K
Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

841
A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
08:04

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

12.6K

Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Meiotic crossovers (COs) are essential for proper chromosome segregation in eukaryotes.
  • ZMM proteins, including ZIP2 and SPO16, are conserved and critical for CO formation in yeast.
  • The conservation of these mechanisms in plants, specifically rice, remained unclear.

Purpose of the Study:

  • To investigate the functions of rice ZIP2 ortholog (OsSHOC1) and SPO16 ortholog (OsPTD) in meiotic CO formation.
  • To elucidate the interactions between OsSHOC1, OsPTD, and other ZMM proteins in rice.
  • To determine the conserved roles of these proteins in plant meiosis.

Main Methods:

  • Characterization of OsSHOC1 and OsPTD gene functions through disruption.
  • Analysis of meiotic recombination intermediates and CO numbers.
  • Protein-protein interaction studies using bimolecular fluorescence complementation and yeast-three hybrid assays.
  • Genetic analysis to determine epistatic relationships.

Main Results:

  • Disruption of OsSHOC1 reduced CO numbers by approximately 17% without affecting synapsis or early recombination.
  • OsSHOC1 interacts with OsPTD, OsZIP4, and OsMSH5, while OsPTD interacts with OsHEI10.
  • OsSHOC1 and OsPTD are required for the loading of other ZMM proteins, and their localization is dependent on OsZIP4 and OsHEI10.
  • OsSHOC1 and OsPTD were found to be epistatic to OsHEI10 and OsZIP4 in CO formation.

Conclusions:

  • OsSHOC1 and OsPTD play essential, conserved roles in promoting class I meiotic CO formation in rice.
  • These proteins form multiple protein complexes with other ZMM proteins (OsZIP4, OsHEI10) to regulate CO formation.
  • The findings reveal a conserved mechanism for CO control in plants, involving protein complex formation and epistatic interactions.