Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Factors Affecting Solubility04:01

Factors Affecting Solubility

36.7K
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Chȃtelier’s principle. Consider the dissolution of silver iodide:
36.7K
Solubility Equilibria03:07

Solubility Equilibria

56.8K
Solubility equilibria are established when the dissolution and precipitation of a solute species occur at equal rates. These equilibria underlie many natural and technological processes, ranging from tooth decay to water purification. An understanding of the factors affecting compound solubility is, therefore, essential to the effective management of these processes. This section applies previously introduced equilibrium concepts and tools to systems involving dissolution and precipitation.
The...
56.8K
Physical Properties Affecting Solubility02:19

Physical Properties Affecting Solubility

26.2K
Solutions of Gases in Liquids
As for any solution, the solubility of a gas in a liquid is affected by the attractive intermolecular forces between solute and solvent species. Unlike solid and liquid solutes, however, there is no solute-solute intermolecular attraction to overcome when a gaseous solute dissolves in a liquid solvent since the atoms or molecules comprising a gas are far separated and experience negligible interactions. Consequently, solute-solvent interactions are the sole...
26.2K
Solubility of Ionic Compounds02:55

Solubility of Ionic Compounds

68.1K
Solubility is the measure of the maximum amount of solute that can be dissolved in a given quantity of solvent at a given temperature and pressure. Solubility is usually measured in molarity (M) or moles per liter (mol/L). A compound is termed soluble if it dissolves in water.
68.1K
Solubility03:00

Solubility

21.0K
Solution, Solubility, and Solubility Equilibrium
A solution is a homogeneous mixture composed of a solvent, the major component, and a solute, the minor component. The physical state of a solution—solid, liquid, or gas—is typically the same as that of the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
In a solution, the solute particles (molecules,...
21.0K
Parallel Resonance01:23

Parallel Resonance

531
The parallel RLC circuit is an arrangement where the resistor (R), inductor (L), and capacitor (C) are all connected to the same nodes and, as a result, share the same voltage across them. The parallel RLC circuit is analyzed in terms of admittance (Y), which reflects the ease with which current can flow. The admittance is given by:
531

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute ischemic stroke during TAVI promptly managed with mechanical thrombectomy: the importance of peripheral interventional skills and multidisciplinary collaboration.

Journal of cardiology cases·2026
Same author

The landscape of antibody production systems: recombinant expression for research, diagnostics and therapy.

Frontiers in bioengineering and biotechnology·2026
Same author

Novel bispecific T-cell engagers overcoming acquired EGFR resistance.

mAbs·2026
Same author

Do Not Let Them Fall-ot: Emerging Role of Transcatheter Therapies in Adult Congenital Heart Diseases.

JACC. Case reports·2026
Same author

Impact of Proportionality of Secondary Tricuspid Regurgitation on Outcomes After Tricuspid Transcatheter Edge-to-Edge Repair.

Circulation. Cardiovascular interventions·2026
Same author

Incidence, Predictors and Outcomes of Bleeding Following Transcatheter Tricuspid Valve Repair: The TriValve Registry.

JACC. Cardiovascular interventions·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jan 22, 2026

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
04:45

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Published on: February 10, 2022

7.8K

Parallelized Microscale Expression of Soluble scFv.

Giulio Russo1, Viola Fühner1, André Frenzel1,2

  • 1Technische Universität Braunschweig, Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Braunschweig, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|July 4, 2019
PubMed
Summary
This summary is machine-generated.

Antibody phage display enables recombinant antibody production. This protocol details parallel microscale production of single-chain variable fragments (scFvs) for efficient screening of antigen-specific binders.

Keywords:
Monoclonal antibody screeningPhage displaySingle-chain fragment variable (scFv)Small-scale antibody production in MTP

More Related Videos

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
12:03

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

34.1K
Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique
10:12

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique

Published on: June 12, 2015

9.5K

Related Experiment Videos

Last Updated: Jan 22, 2026

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
04:45

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Published on: February 10, 2022

7.8K
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
12:03

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

34.1K
Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique
10:12

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique

Published on: June 12, 2015

9.5K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • Antibody phage display is crucial for generating recombinant antibodies for diagnostics, therapeutics, and research.
  • Screening monoclonal binders for antigen specificity is essential after antibody selection.
  • High-throughput methods for producing soluble antibody fragments are needed for efficient screening.

Purpose of the Study:

  • To provide a protocol for parallel microscale production of single-chain variable fragments (scFvs).
  • To facilitate the screening of monoclonal binders for antigen specificity.
  • To enable further assays using produced antibody fragments.

Main Methods:

  • Parallelized microscale production of scFvs.
  • Utilizing microtiter plates for soluble antibody fragment production.
  • Application of antibody phage display technology.

Main Results:

  • A detailed protocol for parallel scFv production.
  • Enabling efficient screening of antigen-specific antibodies.
  • Facilitating downstream applications of antibody fragments.

Conclusions:

  • The described protocol supports high-throughput screening of antibody binders.
  • This method is essential for isolating specific antibodies from phage display libraries.
  • The protocol aids in the development of diagnostic and therapeutic antibodies.