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Updated: Jan 22, 2026

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization
Daniel Some1, Vladimir Razinkov2
1Wyatt Technology Corp., Santa Barbara, CA, USA. dsome@wyatt.com.
Abstract:
We present a review of high-throughput techniques for the characterization and quality control of proteins in the course of purification, evaluation, and formulation, based on static and dynamic light scattering. Multi-angle static light scattering (MALS) in combination with rapid, low-volume UHPLC size exclusion chromatography is effective in characterizing key biophysical properties, while dynamic light scattering (DLS) in high-throughput microwell-plate format provides large quantities of data in a short time to screen many conditions, excipients, cell lines, or candidate biotherapeutics.
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