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Updated: Jan 22, 2026

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
Published on: June 28, 2019
Recent Advances of Molecular Optical Probes in Imaging of β-Galactosidase
Jianjian Zhang1,2, Penghui Cheng2, Kanyi Pu2
1Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, Modern Separation Science Key Laboratory of Shaanxi Province, College of Chemistry & Materials Science , Northwest University , 710127 , Xi'an , China.
Abstract:
β-Galactosidase (β-Gal), as a lysosomal hydrolytic enzyme, plays an important physiological role in catalyzing the hydrolysis of glycosidic bonds which convert lactose into galactose. Moreover, upregulation of β-Gal is often correlated with the occurrence of primary ovarian cancers and cell senescence. Thereby, detection of β-Gal activity is relevant to cancer diagnosis. Optical imaging possesses high spatial and temporal resolution, high sensitivity, and real-time imaging capability. These properties are beneficial for the detection of β-Gal in living systems. This Review summarizes the recent progress in development of molecular optical probes for near-infrared fluorescence (NIRF), bioluminescence (BL), chemiluminescence (CL), or photoacoustic (PA) imaging of β-Gal in biological systems. The challenges and opportunities in the probe design for detection of β-Gal are also discussed.
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