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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Complete Genome Sequence of Serratia marcescens Podophage Parlo

Ryan Bockoven1, Jamie Gutierrez1, Heather Newkirk1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|July 5, 2019
PubMed

Insights

We sequenced the genome of Serratia marcescens podophage Parlo. This phage genome contains a gene for an antirestriction protein, potentially aiding bacterial infection.

Area of Science:

  • Microbiology
  • Genomics
  • Virology

Background:

  • Serratia marcescens is an opportunistic pathogen causing infections in hospitalized patients.
  • This bacterium possesses multiple resistance mechanisms, complicating treatment.
  • Bacteriophages, viruses that infect bacteria, are being explored for therapeutic potential.

Purpose of the Study:

  • To determine the complete genome sequence of the Serratia marcescens-infecting podophage Parlo.
  • To identify genes within the Parlo genome with potential roles in phage-bacterial interactions.

Main Methods:

  • Whole-genome sequencing of the Parlo bacteriophage.
  • Bioinformatic analysis of the Parlo genome sequence.
  • Comparative genomics with related bacteriophages.

Main Results:

  • The full genome sequence of Serratia marcescens podophage Parlo was determined.
  • Parlo shares significant similarity with Erwinia phage PEp14.
  • A gene encoding a 3,764-residue protein, likely a homolog of the antirestriction protein DarB, was identified.

Conclusions:

  • The genome sequence provides a foundation for understanding Parlo's biology.
  • The identified antirestriction protein homolog may play a role in overcoming bacterial defenses.
  • Further research into Parlo and its proteins could inform phage therapy strategies against Serratia marcescens infections.

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