Simultaneous expression of Ia and cytocidal activity by macrophages, and the consequences for antigen presentation

We have investigated whether the interrelationship of Ia expression and cytotoxicity by macrophages, as the two functions if expressed at the same time, might be counterproductive for T-cell development and function. We report that, under some circumstances, there is a clear dissociation of the two activities, as was demonstrated for both in vitro and in vivo conditions. However, the two functions could also be superimposed. Dissociation or superimposition was determined by (i) the nature of the inducing stimulus, and (ii) by the time-span between stimulation and evaluation. It was found that Ia and tumour killing were mainly expressed by the same macrophage population and, as a result, cytolytic activity, when associated with Ia expression, can be directed against T-cell hybridomas in an antigen-specific manner. The physiological relevance of the dissociation or superimposition of Ia expression and tumour killing by macrophages is discussed.