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Cell Culture01:21

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium
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Ejection of cell laden RPMI-1640 culture medium by Electrohydrodynamic method.

Zhang Haiyi1,2, Wang Can3, Jia Ruiwen1

  • 1Faculty of Information Technology, Beijing University of Technology, Beijing, 100124, China.

Biomedical Microdevices
|July 6, 2019
PubMed
Summary

Electrohydrodynamic (EHD) ejection of cell-laden medium shows promise for biomedical applications. This micro-droplet technique achieves high ejection frequencies and maintains high cell viability, indicating potential for precise cell printing.

Keywords:
Cell printingEjection frequencyElectrohydrodynamicHigh speed camera

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Area of Science:

  • Biomedical Engineering
  • Fluid Dynamics
  • Cell Biology

Background:

  • Micro-droplet ejection is crucial for precise sample deposition in biomedicine.
  • Electrohydrodynamic (EHD) systems offer a method for controlled liquid manipulation.

Purpose of the Study:

  • To investigate the electrohydrodynamic (EHD) ejection of cell-containing medium.
  • To characterize ejection behavior at varying voltages and assess cell viability.

Main Methods:

  • Experimental setup utilizing a home-built EHD system with high voltage, nozzle, and collector.
  • High-speed photography and image processing to analyze droplet ejection dynamics.
  • Assessment of human peripheral blood mononuclear cell survival post-ejection.

Main Results:

  • Two stable ejection states were observed: low voltage (few to tens of Hz) and high voltage (up to 1300 Hz).
  • A transitional voltage range exhibited periodic meniscus oscillations and non-uniform droplet ejection.
  • Human peripheral blood mononuclear cells demonstrated survival rates exceeding 79% after EHD ejection.

Conclusions:

  • EHD micro-droplet ejection is a viable technique for depositing cell-laden media.
  • The method supports high-frequency, precise deposition with significant cell viability.
  • EHD ejection presents a promising approach for advanced cell printing applications.