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Related Concept Videos

Flow Cytometry01:23

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Multipotency of Hematopoietic Stem Cells01:19

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Regulation of Hematopoietic Stem Cells01:01

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Differentiation of Common Myeloid Progenitor Cells01:15

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Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
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Nuclear Protein Sorting01:34

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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
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Mitochondrial Protein Sorting01:39

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Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
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Flow Cytometry and Cell Sorting Using Hematopoietic Progenitor Cells.

Sangeetha Vadakke-Madathil1, Lalita S Limaye2, Vaijayanti P Kale2,3

  • 1Icahn School of Medicine at Mount Sinai, New York, NY, USA. sangeetha.vadakke-madathil@mssm.edu.

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|July 6, 2019
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Summary

Flow cytometry, a laser-based technology, rapidly analyzes cell surface antigens and intracellular molecules. This method, including Fluorescent Activated Cell Sorting (FACS), is essential for isolating hematopoietic stem/progenitor cells (HSPCs).

Keywords:
CD34+ progenitor cellsCell sortingFlow cytometryLSK population

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Flow cytometry is a laser-based technology for rapid cell analysis.
  • It measures cell surface antigens and intracellular molecules in diverse cell types.
  • Hematopoietic stem/progenitor cells (HSPCs) are key research subjects.

Purpose of the Study:

  • To describe the general principles of flow cytometry.
  • To detail methods for isolating HSPCs using flow cytometry.
  • To highlight flow cytometry's utility in stem cell research.

Main Methods:

  • Utilizing light scattering properties to determine cell size and internal complexity.
  • Employing fluorescently conjugated antibodies for antigen detection.
  • Applying Fluorescent Activated Cell Sorting (FACS) for cell separation based on fluorescence.

Main Results:

  • Demonstration of flow cytometry's capability for multiparametric single-cell analysis.
  • Successful isolation of HSPCs from heterogeneous cell populations.
  • Validation of flow cytometry as an indispensable tool in stem cell research.

Conclusions:

  • Flow cytometry provides rapid, multiparametric analysis of cells.
  • Detailed methods enable efficient isolation of HSPCs.
  • This technology is crucial for advancing stem cell research and applications.