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Early and late platelet sequestration in different organs during endotoxic shock
A A Al-Sarraf1, J T Christenson, A Owunwanne
1Dept. of Surgery, Faculty of Medicine, Kuwait University, Safat.
Research in Experimental Medicine. Zeitschrift Fur Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie
|January 1, 1988
Summary
In endotoxin-induced shock, sheep showed significant platelet sequestration in lungs, liver, and kidneys. Platelets aggregated and disaggregated in phases, leading to animal death, but not in the brain.
Area of Science:
- * Physiology
- * Immunology
- * Hematology
Background:
- * Endotoxin-induced shock can cause significant physiological derangements.
- * Platelet activation and sequestration are implicated in shock pathophysiology.
- * Understanding platelet behavior during shock is crucial for developing therapeutic strategies.
Purpose of the Study:
- * To investigate the in vivo kinetics and distribution of platelets during endotoxin-induced shock in a sheep model.
- * To determine the specific organs involved in platelet sequestration following endotoxin administration.
- * To characterize the temporal pattern of platelet aggregation and disaggregation during the shock progression.
Main Methods:
- * Indium-111-oxine-labeled platelets were used to track platelet distribution in sheep.
- * Escherichia coli lipopolysaccharide was administered to induce endotoxin shock.
- * Platelet counts were monitored using phase contrast microscopy, and Indium-111-oxine platelet levels were measured in various organs and circulation.
Main Results:
- * Immediate platelet sequestration occurred in the lungs, liver, and kidneys post-endotoxin injection.
- * A decrease in circulating and splenic Indium-111-oxine platelets was observed, correlating with organ sequestration.
- * Platelet counts decreased, and a biphasic pattern of aggregation and disaggregation was noted, preceding animal demise. No brain sequestration was detected.
Conclusions:
- * Platelet sequestration in the lungs, liver, and kidneys is a prominent early event in endotoxin-induced shock in sheep.
- * The observed biphasic platelet behavior suggests a complex dynamic response during shock progression.
- * The brain appears to be spared from significant platelet sequestration in this model, warranting further investigation.