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Updated: Jan 22, 2026

Biofilm Removal Using Carbon Dioxide Aerosols without Nitrogen Purge
Published on: November 6, 2016
Difficulty in removing biofilm from dry surfaces
F Parvin1, H Hu1, G S Whiteley2
1Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia.
Abstract:
Cleaning is fundamental to infection control. This report demonstrates that a Staphylococcus aureus biofilm is significantly more difficult to remove than dried planktonic bacteria. A single wiping action removed >99.9% (>3 log10) of dried planktonic bacteria, whereas only 1.4 log10 of biofilm (96.66%) was removed by 50 wiping actions with a standardized wiping process.
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