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A Regenerative Endodontic Approach in Mature Ferret Teeth Using Rodent Preameloblast-conditioned Medium
Cristina Bucchi1,2, Álvaro Gimeno-Sandig3, Iván Valdivia-Gandur4
1Human Anatomy and Embryology Unit, Department of Pathology and Experimental Therapy, Faculty of Medicine and Health Sciences, Bellvitge Health Science Campus, University of Barcelona, Barcelona, Spain.
Background:
This study evaluated the effectiveness of a regenerative endodontic approach to regenerate the pulp tissue in mature teeth of ferret. The presence of odontoblast-like cells in the newly-formed tissue of teeth treated with or without preameloblast-conditioned medium was evaluated based on morphological criteria.
Materials And Methods:
Twenty-four canines from six ferrets were treated. The pulp was removed, and the apical foramen was enlarged. After inducing the formation of a blood clot, a collagen sponge with or without preameloblast-conditioned medium was placed underneath the cementoenamel junction. The samples were analyzed at the eighth week of follow-up.
Results:
Vascularized connective tissue was observed in 50% of teeth, without differences between groups. The tissue occupied the apical third of the root canals. Odontoblast-like cells were not observed in any group.
Conclusion:
Revitalization of mature teeth is possible, at least in the apical third of the root canal. Further experimental research is needed to produce more reliable outcomes.
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