In Silico Prediction and In Vitro Assessment of Multifunctional Properties of Postbiotics Obtained From Two Probiotic...

Area of Science:

• Microbiology and Metabolomics
• Bioactivity Screening
• Postbiotic Research

Background:

• Probiotics like Lactobacillus casei and Bacillus coagulans are known for health benefits.
• Understanding the bioactive components of these bacteria is crucial for developing functional foods and therapeutics.
• Postbiotics, derived from microbial cells or their components, offer therapeutic potential without the risks of live microorganisms.

Purpose of the Study:

• To characterize the metabolite profiles of Lactobacillus casei CRL 431 and Bacillus coagulans GBI-30.
• To predict the bioactivities of intracellular content (IC) and cell wall (CW) fractions using in silico methods.
• To evaluate the in vitro bioactivities of IC and CW fractions.

Main Methods:

• Global metabolite profiling using Raman spectroscopy.
• In silico prediction of activity spectra for substance (PASS) approach for bioactivity prediction.
• In vitro assays to assess angiotensin-converting enzyme (ACE)-inhibitory, chelating, and antioxidant activities.

Main Results:

• Metabolite profiling identified fatty acids, amino acids, coenzymes, proteins, and amino sugars.
• In silico analysis predicted significant immune-stimulant, anti-inflammatory, neuroprotective, antiproliferative, immunomodulatory, and antineoplastic activities (Pa > 0.7).
• In vitro assays demonstrated high ACE-inhibitory (>90%), chelating (>79%), and moderate antioxidant (22-57 cellular antioxidant activity units) activities for both IC and CW fractions.

Conclusions:

• Lactobacillus casei CRL 431 and Bacillus coagulans GBI-30 are promising sources of postbiotics.
• The IC and CW fractions exhibit multifunctional bioactivities, including ACE inhibition, chelation, and antioxidant effects.
• These findings suggest potential health benefits and therapeutic applications for postbiotics derived from these bacterial strains.