Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

17.2K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.2K
Random and Systematic Errors01:20

Random and Systematic Errors

14.5K
Scientists always try their best to record measurements with the utmost accuracy and precision. However, sometimes errors do occur. These errors can be random or systematic. Random errors are observed due to the inconsistency or fluctuation in the measurement process, or variations in the quantity itself that is being measured. Such errors fluctuate from being greater than or less than the true value in repeated measurements. Consider a scientist measuring the length of an earthworm using a...
14.5K
Systematic Sampling Method01:17

Systematic Sampling Method

12.7K
Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
Systematic sampling is one of the simplest methods...
12.7K
Group Design02:01

Group Design

10.3K
The most basic experimental design involves two groups: the experimental group and the control group. The two groups are designed to be the same except for one difference— experimental manipulation. The experimental group gets the experimental manipulation—that is, the treatment or variable being tested—and the control group does not. Since experimental manipulation is the only difference between the experimental and control groups, we can be sure that any differences between...
10.3K
Propagation of Uncertainty from Systematic Error01:10

Propagation of Uncertainty from Systematic Error

1.4K
The atomic mass of an element varies due to the relative ratio of its isotopes. A sample's relative proportion of oxygen isotopes influences its average atomic mass. For instance, if we were to measure the atomic mass of oxygen from a sample, the mass would be a weighted average of the isotopic masses of oxygen in that sample. Since a single sample is not likely to perfectly reflect the true atomic mass of oxygen for all the molecules of oxygen on Earth, the mass we obtain from this...
1.4K
Factorial Design02:01

Factorial Design

13.7K
Factorial Analysis is an experimental design that applies Analysis of Variance (ANOVA) statistical procedures to examine a change in a dependent variable due to more than one independent variable, also known as factors. Changes in worker productivity can be reasoned, for example, to be influenced by salary and other conditions, such as skill level. One way to test this hypothesis is by categorizing salary into three levels (low, moderate, and high) and skills sets into two levels (entry level...
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of blood flow restriction training on cardiovascular autonomic function in middle-aged and older adults: a meta-analysis.

Scientific reports·2026
Same author

Bound Phenolics Enhance the Antidepressant-Like Effect of Triticale Bran Insoluble Dietary Fiber in Obese Mice: Evidence for the Involvement of Immune Regulation and Microbiota Modulation.

Journal of agricultural and food chemistry·2026
Same author

Lysine acetylome profiling reveals a dual regulatory role in carbon flux redirection and RNA degradation inhibition in <i>Mycobacterium smegmatis</i>.

Frontiers in microbiology·2026
Same author

A deep clustering spatiotemporal framework for real-time urban traffic risk probability estimation.

Traffic injury prevention·2026
Same author

Starches from Different Tiger Nut Varieties: A Comparative Study of Multi-Scale Structural Characteristics, Functional Properties, and In Vitro Digestibility.

Foods (Basel, Switzerland)·2026
Same author

An appendiceal cancer organoid biobank identifies phenotypic evolution and druggable dependencies of peritoneal carcinomatosis.

Developmental cell·2026
Same journal

Tapt1 deficiency in mice impairs pulmonary lipid homeostasis and normal postnatal respiration by targeting ABCA3 for autophagy-lysosomal degradation.

Journal of genetics and genomics = Yi chuan xue bao·2026
Same journal

Gapless comparative genomics reveals effector landscape and adaptive selection signatures in Plasmodiophora brassicae.

Journal of genetics and genomics = Yi chuan xue bao·2026
Same journal

Yorkie/Scalloped-OVOL-Rac1 axis controls insect wing development by promoting cell proliferation.

Journal of genetics and genomics = Yi chuan xue bao·2026
Same journal

Construction of a high-quality indexed EMS mutant library and a pipeline for identifying causal mutations in wheat by whole-exome sequencing.

Journal of genetics and genomics = Yi chuan xue bao·2026
Same journal

Tissue-resolved comparative developmental transcriptomics reveals auxin-associated regulatory shifts underlying divergent fruit tissue origins in pomes and drupes.

Journal of genetics and genomics = Yi chuan xue bao·2026
Same journal

Characterizing selection signatures in coding and noncoding regions of 14,886 cancer genomes.

Journal of genetics and genomics = Yi chuan xue bao·2026
See all related articles

Related Experiment Video

Updated: Jan 22, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.8K

Dissecting PCNA function with a systematically designed mutant library in yeast.

Qingwen Jiang1, Weimin Zhang1, Chenghao Liu2

  • 1Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China; Shenzhen Key Laboratory of Synthetic Genomics and Center for Synthetic Genomics, Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Journal of Genetics and Genomics = Yi Chuan Xue Bao
|July 9, 2019
PubMed
Summary
This summary is machine-generated.

This study created 304 proliferating cell nuclear antigen (PCNA) mutants to understand DNA repair. A key K168A mutant revealed the PCNA-Rad5 interaction is crucial for DNA damage tolerance via template switching.

Keywords:
DNA damage toleranceGenetic interactionHigh-throughputMutagenesisTemplate switching

More Related Videos

Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

8.2K
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.4K

Related Experiment Videos

Last Updated: Jan 22, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.8K
Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

8.2K
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Proliferating cell nuclear antigen (PCNA) is essential for DNA metabolism.
  • Understanding PCNA's role in DNA repair pathways is critical.

Purpose of the Study:

  • To systematically investigate the functional contribution of each residue in PCNA.
  • To identify key interactions and pathways involving PCNA.

Main Methods:

  • Construction and screening of a 304-mutant PCNA library.
  • High-throughput phenotype screening using DNA damaging reagents.
  • Genetic and biochemical analyses of specific mutants, including K168A.

Main Results:

  • Identified five regions of PCNA sensitive to DNA damaging agents.
  • Demonstrated that the K168A mutant disrupts the PCNA-Rad5 interaction.
  • Showed this disruption impairs the DNA damage tolerance (DDT) pathway.

Conclusions:

  • The PCNA-Rad5 interaction is essential for Rad5 function in DDT.
  • PCNA residue K168 is critical for the PCNA-Rad5 interaction in the template switching (TS) pathway.
  • A versatile PCNA mutant library is provided for future research.