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Updated: Jan 22, 2026

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain
Published on: July 11, 2016
[Whole Genome Sequence Determination and Analysis of Bacillus Subtilis Strain CGMCC 12426]
Hui Dong1, Xuan Hao Li1, Jing Lin Chang1
1Key Laboratory of Tianjin Radiation and Molecular Nuclear Medicine,Institute of Radiation Medicine,CAMS and PUMC,Tianjin 300192,China.
Abstract:
Objective To describe the microbiological characteristics of Bacillus subtilis(B. subtilis)CGMCC 12426 and determine and analyze its complete genome sequences.Methods B. subtilis strain CGMCC 12426 genomic DNA sequencing was performed on a single molecule real-time sequencing(SMRT)platform and the annotation was completed in the NCBI Prokaryotic Genomic Annotation Pipeline(pGAP).Results The complete genomic sequences of the released B. subtilis CGMCC 12426 consisted of a 4 138 265-bp circular chromosome and a 74 165-bp plasmid,which resulted in the prediction of 4581 genes including 4222 coding sequences,87 tRNAs,and 30 rRNAs(which included 5S rRNA,16S rRNA,and 23S rRNA).Conclusion The genome sequencing provided a basis for further investigations on the genetic background of B. subtilis and on the metabolic and regulatory mechanisms.
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