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Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa)
V C Carricarte1, G M Bianchini, J P Muschietti
1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Facultad de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina.
The Biochemical Journal
|February 1, 1988
Abstract:
An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase.