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Updated: Jan 22, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
Published on: August 14, 2021
RNA interference and CRISPR: Promising approaches to better understand and control citrus pathogens
Eduardo Henrique Goulin1, Diogo Manzano Galdeano1, Laís Moreira Granato1
1Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera, Km 158, Cordeiropolis, SP, Brazil.
Abstract:
Citrus crops have great economic importance worldwide. However, citrus production faces many diseases caused by different pathogens, such as bacteria, oomycetes, fungi and viruses. To overcome important plant diseases in general, new technologies have been developed and applied to crop protection, including RNA interference (RNAi) and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) systems. RNAi has been demonstrated to be a powerful tool for application in plant defence mechanisms against different pathogens as well as their respective vectors, and CRISPR/Cas system has become widely used in gene editing or reprogramming or knocking out any chosen DNA/RNA sequence. In this article, we provide an overview of the use of RNAi and CRISPR/Cas technologies in management strategies to control several plants diseases, and we discuss how these strategies can be potentially used against citrus pathogens.
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