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Updated: Jan 22, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
Preincubation with a low concentration of methyl-β-cyclodextrin enhances baculovirus expression system productivity
Jinshan Huang1,2, Na Liu1, Xingjia Shen1,2
1Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, 212018, People's Republic of China.
Objectives:
To enhance the productivity of foreign protein in culture cells using baculovirus expression system.
Results:
A low concentration of MβCD, with the optimal application concentration of 0.25 mM and the appropriate preincubation time range from 10 to 120 min, can efficiently enhance expression levels in both the AcMNPV and BmNPV expression systems.
Conclusions:
Preincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity.
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