Related Experiment Video
Updated: Jan 22, 2026
08:24
Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
Published on: November 11, 2008
16.9K
Integrated Robotic Mini Bioreactor Platform for Automated, Parallel Microbial Cultivation With Online Data Handling
Benjamin Haby1, Sebastian Hans1, Emmanuel Anane1
1Institute of Biotechnology, Technische Universität, Berlin, Germany.
SLAS Technology
|July 11, 2019
Summary
This study introduces an automated mini bioreactor platform for parallel cultivations. It enhances bioprocess development by improving data reliability and transferability during scale-up, reducing failure rates.
Area of Science:
- Biotechnology
- Process Engineering
- Bioprocess Development
Background:
- Industrial bioprocess development faces challenges with experimental search space limitations and data relevance from high-throughput screening.
- Current methods often yield data not directly transferable to industrial scale, leading to scale-up failures.
- Sophistication of experiments, including inputs, sensors, and analytics, impacts development efficiency.
Purpose of the Study:
- To present an automated mini bioreactor platform for enhanced bioprocess development.
- To improve the reliability and transferability of experimental data from early-stage development to industrial scale.
- To address the tradeoff between experimental throughput and process control in bioprocess optimization.
Main Methods:
- Development of an automated mini bioreactor platform for milliliter-scale parallel cultivations.
- Integration of online monitoring, advanced feeding strategies, and dual liquid handlers for operation and at-line analysis.
- Implementation of a central database for end-to-end data exchange and integrated control.
- Utilization of a model-based operation algorithm for complex cultivations, scale-down studies, and strain characterization.
Main Results:
- The platform enables parallel cultivations with online monitoring and control, mimicking industrial processes.
- Dual liquid handlers facilitate simultaneous bioreactor operation and at-line analysis.
- Integrated data management and control ensure seamless data flow and device operation.
- Model-based algorithms improve accuracy in complex cultivations and strain characterization, enhancing data reliability.
Conclusions:
- The automated mini bioreactor platform offers a solution for robust bioprocess development.
- It successfully balances experimental throughput with laboratory-scale bioreactor process control and monitoring.
- The platform significantly increases the reliability and transferability of data, mitigating scale-up risks.
Related Concept Videos
01:20Parallel Processing
651
The brain processes sensory information rapidly due to parallel processing, which involves sending data across multiple neural pathways at the same time. This method allows the brain to manage various sensory qualities, such as shapes, colors, movements, and locations, all concurrently. For instance, when observing a forest landscape, the brain simultaneously processes the movement of leaves, the shapes of trees, the depth between them, and the various shades of green. This enables a quick and...
651
01:29Methods for Controlling Microbial Growth
1.6K
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
1.6K
01:27Chemical Agents for Microbial Control
771
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
771
01:28Biological Methods for Microbial Control
802
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
802
01:15Introduction to Statistical Process Control
626
Statistical Process Control (SPC) is a method used to monitor and control quality within processes, particularly in manufacturing and service delivery, by employing statistical methods. SPC aims to distinguish between natural (common cause) variation and variation due to specific changes or events (special cause), allowing for timely improvements and sustained quality. The control chart, a pivotal tool in SPC, visually displays data over time alongside a central line of upper and lower control...
626
01:23Physical Methods for Controlling Microbial Growth: Temperature
971
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
971