An efficient protocol to use feedback-controlling digital microfluidic fluorimetric sensor for detection of mercury...

Mercury ion is a highly toxic anthropogenic pollutants and has serious well-known effects on human. There is an ever-growing demand for convenient detection of mercury driven contaminants in environment, including coastal seawater. However, most of the reported methods are instrument-based and are not easy for portable detection. Our protocol described an efficient Digital Microfluidics method for detecting mercury in coastal seawater samples. It combined the miniaturization/automation potential of digital microfluidics and the sensitivity of fluorescence probe. To overcome a potential risk of driven failure, induced by diversity ion ingredients in seawater, a feedback control loop was included into control system. The method showed satisfied stability and selectivity in Hg sensing under high salinity condition, with the sensitivity of Hg²⁺ at the parts-per-billion level and total testing time of less than 20 s. With the advantages of being fast, amenable to automation and low cost, this protocol is promising for the formation of simple and rapid sensor device, especially for a routine monitoring and emergency detection of Hg/or other metals in coastal waters.