Fluorescence resonance energy transfer in multifunctional nanofibers designed via block copolymer self-assembly

In the present study, we demonstrate the fabrication of multifunctional nanofibers, loaded with CdSe quantum dots (QDs) and sulforhodamine 101 (S101) dye, via the self-assembly process of a polystyrene-block-poly(4-vinylpyridine) (PS-b-P4VP) block copolymer (BCP). The CdSe QDs and S101 dye were simultaneously incorporated in the cylindrical domains, constituted of P4VP blocks, of the self-assembled BCP structure. The cylindrical domains subsequently were isolated as individual nanofibers via the selective-swelling approach. The confinement imposed due to the nano-dimension geometry of the cylindrical domains enabled the QDs and S101 dye to localize within their Förster radius enabling an efficient fluorescence resonance energy transfer (FRET) between them. The mean lifetime of donor emission varied from 4.56 to 3.38 ns with the change in the ratio of S101 dye and CdSe QDs within the nanofibers. Furthermore, using efficiency measurements and the corresponding Förster distances, donor-acceptor distances were determined. Moreover, the kinetics of energy transfer from CdSe QDs to S101 was studied by the Poisson binding model, to understand the interactions between CdSe QDs and S101 dye molecules. The numbers of dye molecules per CdSe QD were determined, by assuming random distribution of S101 dye molecules around the CdSe QDs in the nanofibers. The results showed that the number of dye molecules per QD increased with increasing concentration of dye molecules in the nanofibers. The resulting multifunctional nanofibers could have potential applications in optoelectronics, photonics and sensors which utilize the FRET process.