Related Experiment Video
Updated: Jan 22, 2026

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring
Published on: May 17, 2024
Effect of Spermatic Nuclear Quality on Live Birth Rates in Intracytoplasmic Sperm Injection
Maroua Hachemi1,2, Mustapha Bensaada2,3, Abdelkader Rouabah1
1Faculty of Nature and Life Sciences, Laboratory of Molecular and Cellular Biology, Frères Mentouri University Constantine I, Constantine, Algeria.
Background:
Our study defines the clinical role of sperm DNA damage in the assisted reproductive technology procedure.
Aim:
To investigate if the compaction of chromatin explored added to the analysis of the sperm DNA fragmentation allows obtaining a new indicator for sperm genome quality linked to live birth rate (LBR).
Design:
This was a prospective study, undergoing 101 cycles in the intracytoplasmic sperm injection (ICSI) program.
Materials And Methods:
The sperm DNA fragmentation index (DFI) has been measured with sperm chromatin dispersion examination. The sperm decondensation index (SDI) of chromatin has been measured with aniline blue procedure; with these indexes, a new parameter has been created: DFI × SDI.
Statistical Analysis:
Pearson's correlation coefficient, Student's t-test, and Chi-square test were used. The quantitative variables were described as mean ± standard deviation. Multivariate logistic regressions were performed with live birth as outcome.
Results:
The sperm concentration, motility, and normal morphology were lower when the DFI was high (P = 0.001). The fertilization rates and the number of obtained embryos were not statistically significant different according to the DFI groups. The SDI does not appear to be linked either with the spermatic parameters or with the ICSI parameters. A low DFI seems to be a beneficial factor to obtain a live birth in ICSI procedure (P = 0.064). In case of high DFI, a high SDI allows to obtain a higher LBR than a low SDI.
Conclusion:
The DFI is a good prognostic for a delivery rate in ICSI procedure, and the SDI could be added to DFI to create a new parameter of sperm nuclear quality. This new parameter seems to be linked to LBR.
More Related Videos
09:22Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
Published on: February 9, 2015
09:16U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
Published on: March 28, 2025
Related Concept Videos
Birth Control Methods
Nuclear Stability
To hold positively charged protons together...
Nuclear Fission
Nuclear Fusion
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
Radioactivity and Nuclear Equations
A nuclide of an element has a specific number of protons and...
Sperm Transport
The maturation phase occurs in the epididymis, where sperm...