Related Experiment Video

Updated: Jan 22, 2026

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
07:42

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Published on: June 5, 2014

8.4K

Interleukin-17 and matrix metalloprotease-9 expression in the mycetoma granuloma

Emmanuel Edwar Siddig1,2,3, Ali Mahmoud Mohammed Edris2, Sahar Mubarak Bakhiet4

  • 1The Mycetoma Research Centre, University of Khartoum, Khartoum, Sudan.

Plos Neglected Tropical Diseases
|July 12, 2019
PubMed

Insights

Interleukin-17 (IL-17) and matrix metalloprotease-9 (MMP-9) are key inflammatory markers in mycetoma granulomas. Their expression correlates with disease severity and duration, independent of the causative agent.

Area of Science:

  • Immunology
  • Pathology
  • Dermatology

Background:

  • Mycetoma is a chronic inflammatory disease characterized by grains formed by causative agents.
  • The immunophenotypic characteristics and inflammatory processes in mycetoma granulomas are not fully understood.
  • Interleukin-17 (IL-17) and matrix metalloprotease-9 (MMP-9) are implicated in inflammatory and tissue remodeling processes.

Purpose of the Study:

  • To investigate the expression of IL-17 and MMP-9 in mycetoma granulomas.
  • To determine the correlation between IL-17 and MMP-9 expression and mycetoma disease severity.
  • To assess the relationship between these markers and the causative agents or patient characteristics.

Main Methods:

  • Immunohistochemical evaluation of IL-17 and MMP-9 expression in surgical biopsies from 100 mycetoma patients.
  • Analysis of marker expression in different zones of the granuloma.
  • Statistical analysis to correlate marker levels with disease duration, causative agents, and demographic data.

Main Results:

  • IL-17 was primarily expressed in granuloma Zones I and II, while MMP-9 was mainly found in Zones II and III.
  • MMP-9 expression was higher in bacterial mycetoma (Actinomadura pelletierii, Streptomyces somaliensis) compared to fungal mycetoma (Madurella mycetomatis).
  • MMP-9 levels showed a direct positive correlation with IL-17 levels (p = 0.001) and disease duration (p<0.001).

Conclusions:

  • IL-17 and MMP-9 are significantly expressed in mycetoma granulomas and their levels correlate with disease severity and duration.
  • The findings suggest a role for IL-17 and MMP-9 in the pathogenesis of mycetoma, irrespective of the etiological agent.
  • Further research into these markers could inform therapeutic strategies for mycetoma management.

More Related Videos

Quantification of Immunostained Caspase-9 in Retinal Tissue05:18

Quantification of Immunostained Caspase-9 in Retinal Tissue

Published on: July 25, 2022

1.8K
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells12:38

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Published on: December 18, 2008

11.6K

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.3K
The Extracellular Matrix01:42

The Extracellular Matrix

Overview
88.3K
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.0K
The Bone Matrix01:18

The Bone Matrix

Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...
5.5K
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
5.2K
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
5.0K