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Electrochemical aptasensor for activated protein C using a gold nanoparticle - Chitosan/graphene paste modified
Maryam Hosseini Ghalehno1, Mohammad Mirzaei2, Masoud Torkzadeh-Mahani3
1Department of Chemistry, University of Shahid Bahonar Kerman, Kerman, Iran; Young Research Society, Shahid Bahonar University of Kerman, Kerman, Iran.
A novel electrochemical aptasensor detects activated protein C (APC) using methylene blue. This sensor, built on a modified electrode, offers a sensitive and selective method for APC quantification in biological samples.
Area of Science:
- Electrochemistry
- Biosensors
- Biochemistry
Background:
- Activated protein C (APC) plays a crucial role in coagulation.
- Accurate detection of APC is vital for diagnosing and managing related diseases.
- Existing detection methods may lack sensitivity or simplicity.
Purpose of the Study:
- To develop a selective and simple electrochemical aptasensor for APC detection.
- To utilize methylene blue as a redox indicator for signal transduction.
- To establish a sensitive platform for APC quantification in biological matrices.
Main Methods:
- Fabrication of a carbon paste electrode modified with gold nanoparticle-chitosan/graphene (AuNPs-Chi/Gr).
- Covalent immobilization of activated protein C aptamer (APC-apt) onto the modified electrode surface.
- Electrochemical characterization using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
- Quantification of APC using differential pulse voltammetry (DPV) based on methylene blue signal changes.
Main Results:
- Successful construction and aptasensor immobilization confirmed by CV and EIS.
- A linear detection range for APC from 0.1 ng·mL⁻¹ to 40 μg·mL⁻¹ was achieved.
- A low detection limit of 0.073 ng·mL⁻¹ for APC was obtained.
- The aptasensor demonstrated effective determination of APC in human serum samples.
Conclusions:
- The developed electrochemical aptasensor provides a sensitive and selective method for APC detection.
- The AuNPs-Chi/Gr composite material enhances sensor performance and aptasensor stability.
- This strategy holds potential for detecting other proteins in complex biological samples.
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