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Prostaglandin synthesis by the cochlea.

R Kawata1, Y Urade, M Tachibana

  • 1Department of Otolaryngology, Kyoto Prefectual University of Medicine, Japan.

Prostaglandins
|February 1, 1988
PubMed
Summary

This study investigated prostaglandin synthesis in the gerbil cochlea. Prostaglandins are produced both from external and internal sources, with PGE2 being most prevalent in the cochlear nerve and organ of Corti.

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Area of Science:

  • Otoacoustic emissions and inner ear physiology
  • Biochemistry and molecular biology
  • Neuroscience and audiology

Background:

  • Prostaglandins (PGs) play crucial roles in auditory function and inflammation.
  • Understanding PG synthesis in the cochlea is vital for auditory research.

Purpose of the Study:

  • To investigate the exogenous and endogenous synthesis of prostaglandins in the Mongolian gerbil cochlea.
  • To identify the specific types and locations of PG synthesis within the cochlea.

Main Methods:

  • In vitro incubation of gerbil cochlea with [3H]-arachidonic acid (AA).
  • Radioimmunoassay (RIA) to quantify PG levels.
  • Indomethacin to inhibit PG synthesis.
  • Microwave irradiation to inactivate endogenous enzymes.
  • Dissection of cochlea into organ of Corti + modiolus (OC + M), lateral wall (LW), and cochlear nerve (CN) for regional analysis.

Main Results:

  • Exogenous synthesis of PGF2 alpha, 6-keto PGF1 alpha, PGE2, TXB2, and PGD2 was confirmed, inhibited by indomethacin.
  • Endogenous synthesis of PGD2, PGE2, PGF2 alpha, and 6-keto PGF1 alpha was detected in non-microwave-treated cochlea homogenates.
  • PGE2 was the predominant PG in the cochlear nerve (CN) and organ of Corti + modiolus (OC + M).
  • PG levels were relatively uniform across the lateral wall (LW).
  • Thromboxane B2 (TXB2) levels were below the RIA detection limit.

Conclusions:

  • The gerbil cochlea actively synthesizes various prostaglandins from both exogenous and endogenous sources.
  • PGE2 is a major prostaglandin in the neural and sensory-unctional regions of the cochlea.
  • These findings provide insights into the local biochemical environment of the cochlea and its potential role in auditory processes.

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