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Updated: Jan 22, 2026

A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood
Published on: August 15, 2025
Optimized centrifugation preparation of the platelet rich plasma: Literature review
1Department of Maxillofacial and Plastic Facial Surgery, Trousseau Hospital, CHU Trousseau, Avenvue de la république, 37170 Chambray-lès-Tours, France; School of medicine, University of François Rabelais, 370 Tours, France.
Abstract:
Nowadays, the Platelet Rich Plasma (PRP) is frequently used for its therapeutic effects on wound healing, and this due to secretion of many growth factors. However, no standardized procedure has been set up. The aim of this article is to check the various preparations (centrifugation time and speed). This review recorded all the international articles published between 2007 and 2018, for which the assessment criteria were the platelet concentration and/or the growth factor release rate. A multitude of protocols has been looked at with a simple or double centrifugation. All of them have shown an increase in the platelet concentration allowing a therapeutic effect. However, when the centrifugation force is extended, platelets can possibly be altered. The diversity of methods can be linked to the use of various centrifuges. A procedure with simple centrifugation would be a good compromise for the day-to-day use of the PRP in surgery.
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