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Validation and Characterization of Maize Multiple Disease Resistance QTL
Lais B Martins1, Elizabeth Rucker2, Wade Thomason2
1Dept. of Crop Science, North Carolina State University, Box 7620, Raleigh, NC 27695.
G3 (Bethesda, Md.)
|July 14, 2019
Summary
This study validated multiple-disease resistance quantitative trait loci (QTL) in maize, confirming two loci confer resistance to Southern Leaf Blight, Northern Leaf Blight, and Gray Leaf Spot.
Area of Science:
- Plant Pathology
- Genetics
- Agronomy
Background:
- Southern Leaf Blight, Northern Leaf Blight, and Gray Leaf Spot are major maize foliar diseases.
- Quantitative trait loci (QTL) for resistance have been identified, with some co-localizing as multiple disease resistance (MDR) QTL.
Purpose of the Study:
- To independently validate previously identified MDR QTL for maize foliar diseases.
- To refine estimates of allele effects for maize disease resistance.
Main Methods:
- Developed twelve F2:3 family populations segregating for targeted QTL.
- Assessed populations for resistance to three diseases in replicated trials.
- Genotyped individuals using markers linked to disease resistance.
Main Results:
- 16 out of 44 tested QTL were validated at the same genomic locations.
- Significant phenotypic correlations (0.2-0.3) were observed between resistance to the three diseases.
- Two MDR QTL were identified conferring resistance to all three diseases.
Conclusions:
- Independent validation confirmed several important MDR QTL for maize foliar diseases.
- This research highlights the utility of MDR QTL in breeding for broad-spectrum disease resistance.
- Two novel MDR QTL provide targets for enhancing maize disease resistance.
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