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Updated: Jan 22, 2026

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
Published on: January 19, 2020
Prediction of the size of electroformed giant unilamellar vesicle using response surface methodology
Salah Eddine Ghellab1, Wei Mu1, Qingchuang Li1
1State Key Laboratory of Urban Water Resource and Environment, School of Chemical Engineering and Technology, Harbin Institute of Technology, 92West Da-Zhi Street, Harbin 150001, China.
Abstract:
The production of giant unilamellar vesicles (GUVs) with specific size and structure has been a challenge on the design of quantitative biological assays in cell-mimetic micro-compartments. In this study, the effect of electroformation parameters (electric potential, frequency, and temperature) on the size of GUVs was investigated. Using response surface methodology based on Box-Behnken design, GUVs from neutral, positive and negative charges were formulated. The average diameter of GUVs was determined for each formulation. The acquired data of these GUVs were successfully fitted with quadratic regression models. These models were applied to visualize the parameters for ideal GUVs with wanted diameters by the obtained phase diagrams. These results show that response surface methodology can be used to estimate the electroformation parameters for specifically sized GUVs.
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